HOLODETECT FLOW IMAGING

AUTOMATE MICROBE AND PARTICLE MEASUREMENTS IN LIQUIDS

YOU CAN'T IMPROVE WHAT YOU CAN'T MEASURE

AUTOMATED CULTURE CHARACTERIZATION

Laboratory analysis of various cell cultures or other suspension samples can be tedious. Manual sample preparation and cell counting may introduce high variability and lower consistency. Holodetect lab devices enable full automation of the analysis pipeline with optional autosampler integration for automated analysis of multiple samples.

FLUORESCENCE MICROSCOPY

Our HiRes+Fluor system combines an integrated fluorescence microscope with automatic cell counting based on fluorescence. It provides fluorescence intensity measurements at both the cell and sample level, allowing accurate estimation of the chlorophyll content. The system also supports labelled measurements using species- or function-specific dyes (e.g. distinguishing live and dead cells).

AI BASED CLASSIFICATION

The AI-based system offers advanced object detection, segmentation, and classification. With flexible retraining capabilities, it can classify and count different species, in monocultures and is multi-species cultures as well. The few-shot learning module allows for rapid on-site retraining, ensuring quick adaptation to new species or conditions.

CONTAMINATION DETECTION 

Using AI classifiers trained on combined morphological and fluorescence data, Holodetect
detects contaminants (e.g., Chlorella, Rotifers etc.) at levels as low as 0.5%,
It provides early alerts within 12–24 h of contamination onset.

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SOLUTIONS & SERVICES

The cornerstone of operational efficiency is the continuous monitoring and analysis of key operational parameters. This is also the foundation of the successful scale-up of microalgae production. Holodetec’s devices will provide this information. Whether it’s an inline sensor or a laboratory desktop device, their advanced volumetric and fluorescence imaging technologies, combined with user-trainable AI models, enable full automation of microscopy analysis tasks.
LABORATORY AUTOMATION, AUTOMATED SAMPLE ANALYSIS

Laboratory analysis of various cell cultures or other suspension samples can be tedious. Manual sample preparation and cell counting may introduce high variability and lower consistency. Holodetect lab devices enable full automation of the analysis pipeline with optional autosampler integration for automated analysis of multiple samples.

INDUSTRIAL INLINE SOLUTIONS AND INTEGRATION


Holodetect instruments can be interfaced with existing or under development industrial production processes (e.g. food processing, microalgae culturing, water quality monitoring). We are ready to implement custom sampling and measurement data relaying to the control loop in custom systems.

AI BASED CULTURE ANALYSIS AND CLASSIFICATION

Object detection, segmentation, classification, and overall culture analysis are supported by deep learning models.
These models can be retrained using the built-in Quick AI Trainer (QAIT) module for different cell morphologies. 
The user can define arbitrary number of classes, enabling cell counting and classification in mixed/multi-species cultures.

HOLODETECT PRODUCTS

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Holodetect HiRes/LVol

High Resolution Digital Holographic Microscope

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Holodetect HiRes/LVol + Fluor3

High Resolution Digital Holographic Microscope with Multispectral Flourescence Microscope

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Holodetect SmartSampler

Automated Sampling and Sample Preparation with Multispectral OD, Turbidity and Fluorescence Sensors

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TECHNOLOGY

Holodetect devices are automatic volumetric microscopes featuring flow-through measurement cuvettes with depths ranging from 100 to 10,000 microns to accommodate different magnification needs. Leveraging the power of digital holographic microscopy, the device captures the entire sample depth in a single image, eliminating the need for optical refocusing as it is done numerically post-recording. This technology allows for reconstructing all sample objects from the recorded hologram with minimal sample preparation.

DIGITAL OBJECT FOCUSING

Once the hologram has been recorded, it can be digitally reconstructed at different depths. The following examples show how the object comes into focus when the hologram is propagated at different depths. This allows us to efficiently capture an entire volume of space and quickly and accurately identify objects at different depths.
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Nematode
Large Volume DHM
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Rotifer
Large Volume DHM
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Spirulina
Large Volume DHM
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Haematococcus
High Resolution DHM
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Scenedesmus
High Resolution DHM
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Yeast
High Resolution DHM
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AI BASED ANALYSIS

The analysis of reconstructed images is powered by advanced artificial intelligence. These AI models are highly adaptable and can be trained to identify various species and objects within the sample accurately. Additionally, our holographic microscope can be integrated with fluorescent microscopy to enhance classification precision and conduct a variety of fluorescence-based measurements. This synergy between technologies ensures comprehensive sample analysis with unparalleled accuracy.

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NEWS

Holodetect Instruments showcased its latest developments at AlgaEurope 2025 in Riga. Among other novelties, a new, versatile low-cost inline digital holographic microscope, and new sampler/diluter that connects to multiple reactors and doubles as an inline turbidity and biomass sensor have made their debut. We are grateful for all the interest and support we received at the conference. We are looking forward to meeting you again in Malta in 2026!

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ABOUT US

Holodetect Instruments is a technology-driven company specializing in advanced inline monitoring solutions for algae cultivation and bioprocessing. We develop and manufacture digital holographic and fluorescence microscopes combined with AI-enabled analytics to help operators see and act on their processes in real-time. Our systems deliver continuous, label-free imaging of microorganisms in flow, with optional fluorescence channels for species-specific detection. Using our Quick AI Trainer (QAIT), customers can rapidly adapt models to their own strains and processes, ensuring high accuracy in diverse conditions. Holodetect’s instruments enable higher productivity, fewer contamination losses, and optimized resource use in photobioreactors, fermenters, and water utilities.

Contact us

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CONTACT

Chief Executive Officer: Dr. László Nemes
Address: H-1111 Budapest, Bartók Béla út 28A

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